Biomarker Laboratory

CHDR houses and operates a state-of-the-art cellular laboratory and can handle fresh samples collected from both healthy subjects and patients to measure a wide variety of cellular responses. Throughout 2017, CHDR built the new cellular laboratory almost entirely from scratch. The facilities were upgraded to a dedicated, fully equipped cell culture lab with sufficient space for four technicians. An additional flow cytometer was acquired, the number of flow hoods and incubators was increased, and new equipment was purchased, including a fluorescence microscope, a stereo microscope,and ELISA and ELISPOT readers. Combined with Bedside laboratory testing, magnetic cell sorter, our facilities are now well equipped for studying drug effects at the molecular and cellular levels. Moreover, CHDR’s R&D laboratory staff increased from two members to eight.

Methods

Cell isolation/sorting
  • Magnetic sorting (RoboSep)
Immunophenotyping
  • FACS (MACSQuant)
  • Cell surface markers
  • Intracelluar cytokines
  • Phosphoprotein profiling
Cell incubations / cultures
  • Primary cells (whole blood, PBMCs)
  • Intended pharmacology - In vitro and ex vivo drug effects
  • Unintended pharmacology - Fcgamma-mediated effects (Abs), contamination, endosomal TLRs (oligonucleotides)
  • Cell culture lab
  • Flow cytometer
  • Flow hoods
  • Fluorescent microscopy
  • ELISA
  • Magnetic cell sorter
  • ELISPOT reader

Biomarkers in recent studies: examples

Dermatology research:
-Disease areas: psoriasis, atopic dermatitis, acne, HPV-mediated conditions
-Pathways / mechanisms of action:
•TLR7/8/9 (ex vivo)
•TLR7 (in vivo imiquimod challenge)
•T cell costimulation
•Antimicrobial peptides
•Immunostimulation by antisense oligonucleotides (ex vivo)
Vascular medicine research:
-Disease areas: rheumatoid arthritis, atherosclerosis, pulmonary fibrosis
-Pathways / mechanisms of action:
•Inflammasome
•T cell costimulation
•p38 MAPK
•TLR4
•Serum amyloid P
CNS research:
-Disease areas: PD, MS, HD, AD (neurodegeneration), depression
-Pathways / mechanisms of action:
•TLR9
•Inflammasome
•Phosphokinases
•Cholinergic pathways
•In vivo myelin turnover
•Mitochondrial function (relevant for neurodegenerative diseases)
Contact CHDR