CHDR houses and operates a state-of-the-art cellular laboratory and can handle fresh samples collected from both healthy subjects and patients to measure a wide variety of cellular responses.

State-of-the-art laboratory

CHDR's cellular laboratory was rebuilt in 2017 almost entirely from scratch. The facilities were upgraded to a dedicated, fully-equipped cell culture lab with space for four technicians. We also acquired an additional flow cytometer, increased the number of flow hoods and incubators, and purchased new equipment, including a fluorescence microscope, a stereo microscope, and ELISA and ELISPOT readers. Combined with bedside laboratory testing and a magnetic cell sorter, our facilities are fully equipped for the study of drug effects at the molecular and cellular levels. CHDR’s R&D laboratory team has also been expanded in recent years.

Have a look inside our biomarker lab

Facilities and equipment

  • Cell culture lab
  • Flow cytometer
  • Flow hoods
  • Fluorescent microscopy
  • ELISA
  • Magnetic cell sorter
  • ELISPOT reader

Capabilities

Cell isolation / sorting

  • Magnetic sorting (RoboSep)

Immunophenotyping

  • Flow cytometry (MACSQuant)
  • Cell surface markers
  • Intracelluar cytokines
  • Phosphoprotein profiling

Cell incubations / cultures

  • Primary cells (whole blood, PBMCs)
  • Intended pharmacology – in vitro and ex vivo drug effects
  • Unintended pharmacology – Fc-gamma mediated effects (Abs), contamination, endosomal TLRs (oligonucleotides)

Biomarkers in recent studies: examples

Dermatology research

  • Disease areas: psoriasis, atopic dermatitis, acne, HPV-mediated conditions
  • Pathways / mechanisms of action:

    · TLR7/8/9 (ex vivo)

    · TLR7 (in vivo imiquimod challenge)

    · T-cell costimulation

    · Antimicrobial peptides

    · Immunostimulation by antisense oligonucleotides (ex vivo)

Vascular medicine research

  • Disease areas: rheumatoid arthritis, atherosclerosis, pulmonary fibrosis
  • Pathways / mechanisms of action:

    · Inflammasome

    · T-cell costimulation

    · p38 MAPK

    · TLR4

    · Serum amyloid P

CNS research

  • Disease areas: Parkinson's, MS, Huntington's, Alzheimer's (neurodegeneration), depression
  • Pathways / mechanisms of action:

    · TLR9

    · Inflammasome

    · Phosphokinases

    · Cholinergic pathways

    · In vivo myelin turnover

    · Mitochondrial function (relevant for neurodegenerative diseases)

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