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Methods & Biomarkers

Isolation of primary human cells

CHDR offers a fully equipped cell culture laboratory, with flow hoods, CO2 incubators and a magnetic cell sorter, run by experienced bioanalytical staff.

Summary

CHDR has extensive experience in the isolation of primary human cells for downstream analysis of drug effects. Since sample handling can have a large effect on cellular behaviour, optimisation of cell isolation and storage is key. We have three different standardised procedures in place for the isolation of peripheral mononuclear blood cells, and we select one of these three for application in a clinical study, based on the intended use of the isolated cells. The availability of a flow cytometer and expert technical staff enables quality control of isolated cells, in terms of yield, purity and viability. The in-house laboratory at CHDR is also equipped with STEMCELL Technologies’ RoboSep magnetic cell separator. Cell sorting is frequently applied in our clinical studies for focused analysis of drug effects on target cells.

Inflammatory challenges: A series of in vivo, ex vivo, and in vitro tools to induce inflammation in healthy volunteers and cells

Inflammation is a primary pathogenic pathway in a wide range of diseases, including rheumatoid arthritis, Crohn’s disease, cardiovascular disease, and neuropsychiatric disorders. To study the effect of compounds that target inflammatory pathways in the early phases of drug development, CHDR has developed a series of in vivo, ex vivo, and in vitro tools to induce inflammation in healthy subjects and in cells.

Inflammatory challenges

Cell cultures and incubations/challenges: The isolation of primary human cells for downstream analysis of drug effects

At CHDR, we have the expertise, laboratory capacity, and access to human samples needed to develop customised biomarkers to answer specific research questions.

Cell cultures and incubations/challenges

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