Summary
CHDR’s in-house laboratory features a MACSQuant flow cytometer from Miltenyi Biotec, which allows immunophenotyping of target cells. We frequently apply this technique in clinical studies, since demonstrating proximal pharmacological activity can be key to a successful drug development programme. Flow cytometry also plays a vital role in CHDR’s in vitro translational activities, in experiments studying proximal pharmacological effects of investigational compounds in primary human immune cells of healthy donors or specific patient populations. We mainly apply immunophenotyping in the areas of cellular stress (apoptosis, mitochondrial function), immunomodulation (T cell activation and proliferation assays, innate immune challenges), receptor expression and occupancy, and quality control of isolated cell subsets.
