Summary

CHDR’s in-house laboratory is equipped with Miltenyi’s MACSQuant flow cytometer, which allows immunophenotyping of target cells. CHDR applies frequently this technique in clinical studies, since demonstration of proximal pharmacological activity can be key to a successful drug development program. Moreover, flow cytometry is important for CHDR’s translational activities in vitro, in experiments in which proximal pharmacological effects of investigational compounds are studied in primary human immune cells of healthy donors or specific patient populations. CHDR mainly applies immunophenotyping in the areas of cellular stress (apoptosis, mitochondrial function), immunomodulation (T cell activation and proliferation assays, innate immune challenges), receptor expression and occupancy, and quality control of isolated cell subsets.